Emory Integrated Genomics Core
The EIGC, one of the Emory Integrated Core Facilities, is a CLIA-certified (CLIA:11D1086150) laboratory located on the 7th floor of the Woodruff Memorial Research Building, with 2400 square feet of dedicated wet-lab space.
The EIGC’s laboratory areas include dedicated pre- and post-PCR spaces. Two chemical fume hoods are also located within the space. The EIGC has 500 square feet of dedicated office adjacent to the laboratory space on the 7th floor of the Woodruff Memorial Research Building, which provides for computational services, meeting customers, and weekly EIGC team meetings.
The EIGC is composed of three divisions: CLIA, Research, and Custom Cloning. Each division provides specialized genomics research services to Emory investigators. A Thermo Scientific Nautilus Laboratory Information Management System (LIMs) is hosted at Emory and all data is stored on-site in a HIPAA secure zone.
This LIMS provides the foundation for EIGC workflows and fosters collaboration across the Emory campus and with external consortia. The EIGC also closely coordinates with the Emory Integrated Computational Core, which is located on the 7th floor of the Woodruff Memorial Research Building, adjacent to the EIGC, to provide computational and bioinformatics services for Emory investigators.
The EIGC infrastructure and services include:
DNA/RNA Extraction, Genotyping, and Gene Expression
Illumina HiScan Reader. A high performance microarray scanner required to read many of the newer high density Illumina Infinium microarrays. Analyses of whole genome SNP copy number, methylation and gene expression arrays for human and model organisms are supported with this instrument.
nanoString nCounter Technology. A highly sensitive, enzyme-free, molecular counting platform for determining gene expression or target enrichment across a predefined panel of genomic regions. The EIGC assists investigators with project planning, sample preparation, and coordination of nCounter services across a number of outside providers.
Bionano Saphyr. An optical genome scanning device which scans NanoChannel arrays in order to image very long, single DNA molecules. These images make it possible to discover and characterize structural variation that is typically difficult to map using standard short read next-generation sequencing strategies.
10X Genomics Chromium Controller. An automated system that allows one to encapsulate samples into hundreds to tens of thousands of uniquely addressable partitions in minutes, each containing an identifying barcode for downstream analysis. Supports a wide variety of assays when combined with Illumina sequencing that include: Single Cell Gene Expression, Single Cell Immune Profiling, Single Cell Copy Number Variant Detection, Single Cell ATACseq, Genome sequencing, Exome sequencing, de novo Assembly of genomes.
1CellBio InDrop System. A single-cell, high-resolution transcriptomics analysis platform that provides enhanced experimental control, more actionable information and a lower overall cost per result compared to other existing platforms. Supports a wide variety of tissue types and applications to address questions that include cancer, immunobiology, and developmental biology. The platform has a high encapsulation rate (>90%) with low doublets with flexible reagents and protocols to support a wide variety of single cell assays.
Invitrogen Countess. A digital cell counter and viability instrument.
Fluidigm AccessArray. A liquid handling instrument that is primarily used for targeted enrichment of specified genomic regions for Illumina sequencing.
Two Magnetic Particle Processors. One Kingfisher Flex and one MagMax provide for automated extraction of RNA from FFPE tissue in 96-well format and DNA from FFPE tissue, fresh or frozen tissue, blood, blood products, saliva, urine, and cell cultures in 24-well or 96-well format.
Tecan EVO150. Used to perform pre-PCR routine liquid transfers, such as the transfer of nucleic acids from individual vials into the 96-well storage and amplification plates required by downstream applications.
Beckman Biomek NX Automation Workstation. Performs all post-PCR aspects of liquid handling including pipetting, dilution, dispensing, and integrations.
Beckman SPRI-TE robot. For hands-free preparation of up to 10 next generation sequencing libraries every 12 hours.
Tecan Infinite M200 Pro. Quantitates nucleic acids using either PicoGreen or RiboGreen fluorescence protocols.
Agilent Fragment Analyzer (48/96 wells). A is a parallel capillary electrophoresis instrument designed to speed nucleic acid fragment analysis and quality control before moving onto downstream applications.
Agilent 2100 Bioanalyzer. A lab on a chip platform allows for rapid quantification of nucleic and proteomic samples, while providing information about the size distribution of the fragments.
Agilent 2200 TapeStation. Provides simple, fast, and reliable electrophoresis of DNA, RNA, and proteins.
Eight Thermal Cyclers. Five Applied Biosystems 9700 Peltier-driven thermal cyclers, two MJ BioRad thermal cyclers, and one BioRad Tetrad with four 96-well gradient capable blocks.
Applied Biosystems 3130. A multi-color fluorescence-based DNA analysis system using the technology of capillary electrophoresis with 16 capillaries operating in parallel. This fully automated system separates amplified fragments of varying sizes for analysis of short tandem repeats (STRs) among individuals and extended pedigrees as well as sequencing.
Applied Biosystems 7900HT. Is a real-time quantitative PCR system that combines 96- and 384-well plate compatibility with fully automated robotic loading. Key applications include gene expression quantitation and the detection of single nucleotide polymorphisms (SNPs) using the fluorogenic 5' nuclease assay.
Covaris E210 Adaptive Focusing Instrument. Employs ultrasonic pulses to uniformly shear nucleic acids, in a multi-sample format with walk away operation. The instrument can also be used to automate lyses of difficult samples (like mouse tail), shear proteins, and form lipid vesicles.
Non-instrument computers - There are currently one MacPro tower, two MacBook Pros, two iMacs, three Dell Precision Workstations with 64-BIT OS, and five Dell PCs.
Next Generation Sequencing:
One Illumina MiSeq instrument. A fully integrated next generation sequencing platform capable of generating between 540 Mb (~4 hours) up to 8.5 Gb (~39 hours) of raw sequence. Some applications of this platform include targeted sequencing from complex eukaryotic genomes or cancer tumors, microbial whole-genome sequencing, 16S rRNA sequencing for microbiome studies, and sequencing of bisulfite treated DNA for assessing methylation. Samples preparation is rapid and samples can be multiplexed with sequence tags.
One Illumina NextSeq 550 instrument. A fully integrated next generation sequencing platform capable of generating between 16GB to 120GB in instrument runs that range between 11 to 29 hours per experiment. Some applications of this platform include sequencing single cell libraries, targeted sequencing from complex eukaryotic genomes or cancer tumors, microbial whole-genome sequencing, and sequencing of bisulfite treated DNA for assessing methylation.
Large-Scale Next Generation Sequencing. The EIGC has adopted an innovative business model whereby large-scale next-generation sequencing is outsourced to other academic and commercial entities, with the ultimate goal of obtaining the lowest cost, highest quality, and fastest turn-around for our customers. Academic partners include the Genomic Services Laboratory at HudsonAlpha and genomics core facilities at the University of Georgia, Georgia Institute of Technology, and New York University. Commercial companies include: NovaSeq, Akesogen, Otogenetics, and BGI. We have the flexibility to pursue sequencing projects with any outside provider that provides competitive pricing, rapid turn-around time, and high-quality data. In effect, the EIGC acts as a sequencing service broker, whereby we compete companies against each other to obtain the best pricing and service for Emory investigators.