Emory Integrated Lipidomics Metabolomics Core
The Emory Integrated Lipidomics Metabolomics Core, run by Dr. Eric Ortlund, is located within the O. Wayne Rollins Research Center and has 650 feet of wet lab space.
The EILC houses an AB Sciex QTrap5500 enhanced high performance hybrid triple quadrupole/ linear ion trap LC/MS/MS mass spectrometer with mass range of m/z 5 to 1250 in triple quadrupole mode, and 5-1000 in LIT mode.
The instrument is paired with an ExionLC AC HPLC/UHPLC system with an ExionLC column oven and autosampler along with a computer workstation running LipidView software (ABSciex).
The EILC houses a state-of-the-art IDX Orbitrap Fusion Tribrid Mass Spectrometer paired with an Ultimate 3000 HPLC for lipidomics and metabolomics workflows. For sample processing, the EILC uses a Biotage robot for capable of automatic sample extraction and preparation.
Minor equipment includes three -80 °C freezers, nitrogen evaporators, SPE cassette manifolds for specialized lipid extractions, a table top centrifuge, a fume hood, rockers, and multisample vortexers.
AB SciEx QTrap5500. This mass spectrometer is paired with an ExionLC AC HPLC/UHPLC system with an Exion LC column oven and autosampler along with a computer workstation running LipidView Software (AB SciEx). This resource will be used to support targeted lipidomics analyses.
IDX Orbitrap Fusion Tribrid Mass Spectrometer. This mass spectrometer is paired with an Ultimate 3000 HPLC. This resource will to supports lipidomics and metabolomics workflows.
Biotage Extrahera. This automated robotic system supports Supported Liquid Extraction (SLE), Solid Phase Extraction (SPE), Phospholipid Depletion (PLD), and Protein Precipitation (PPT) based methods, in plate or column formats.