Emory Mouse Transgenic & Gene Targeting Core
Emory University
Contact: Karolina Nitsche, PhD
Phone: 404-712-8372
The Emory Mouse Transgenic and Gene Targeting Core (TMF) provides state-of-the-art equipment and expertise for making genetically engineered mouse models, moving alleles on or off campus, or cryopreserving existing mouse strains.
The TMF is located in the vivarium on the ground floor of Emory Health Sciences Research Building 1 (HSRB1). The laboratory space includes a fully equipped tissue culture suite dedicated to mouse embryonic stem (ES) cell culture, a microinjection/embryo manipulation space, and a surgical suite. The TMF laboratory also includes a molecular biology space and cryopreserved embryo/sperm archival space. The animal holding/breeding room is located within the HSRB1 vivarium and is operated by the Division of Animal Resources (DAR).
In total, the TMF core facility is fully equipped to perform all experimental procedures associated with the production of gene-edited mice, as well as auxiliary procedures that include rederivation and cryopreservation services and custom mouse needs.
If you use the TMF for your research, please acknowledge the TMF in your publications and include its RRID (RRID:SCR_023535).
Microinjection/Gene Editing (V206)
Microinjection Station #1:
- Leica DMi8 Inverted microscope with Phase contrast and DIC optics
- Narishige manual micromanipulators
- Eppendorf microinjectors
- Eppendorf FemtoJet 4i transjector
- Prime Tech PMM6G Piezo Impact Drive
- Brook Industries heating/cooling Thermal Stage System
Microinjection Station #2:
- Leica DMi8 Inverted microscope with Phase contrast and DIC optics
- Narishige manual micromanipulators
- Eppendorf microinjectors
- Eppendorf FemtoJet 4i transjector
- Brook Industries heating/cooling Thermal Stage System
Embryo collection, manipulation, and rederivation (V206)
- Leica M80 Binocular Stereo Microscope with heating stage (x2)
- Leica M60 Binocular Stereo Microscope
- Panasonic CO2 Incubator (x2)
- Sutter P-1000 Micropipette puller
- BTX Electroporation system
ES Cell Culture (V208)
- Nuaire Class II Biological Safety Cabinet
- ThermoScientific centrifuge
- Eppendorf 5430R Refrigerated Centrifuge
- Panasonic CO2 Incubator
- Leica DMi1 Inverted Microscope
- BioRad Electroporation Unit
Sperm/Embryo Cryopreservation (V202, V206, V208)
- FTS Bio-Cool Rate Freezer BC-V-40A
- Locator 4 Liquid Nitrogen Dewar
- MVE Liquid Nitrogen Dewars (x11)
- CEROS-2 Animal Sperm Analyzer
Molecular Biology (V209)
- Ohaus Explorer Analytical Balance
- Eppendorf Centrifuge 5415 C
- Thermo Scientific Proflex 3X32-WELL PCR System
- ABI 9700 thermal cycler (x2)
Visit our website to learn more about TMF at Emory University »
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