Proteomics and Metabolomics Mass Spectrometry Facility
University of Georgia
The Proteomics and Metabolomics Mass Spectrometry (PAMMS) Facility at the University of Georgia is equipped with a wide range of mass spectrometers and analytical equipment for the mass spectral analysis of compounds ranging from small organic compounds to peptides and proteins.
The facility also has a Bruker Autoflex and Autoflex III MALDI-TOF mass spectrometer for quick analysis of tryptic digests of pure proteins. The facility offers in-gel digestion and subsequence analysis for protein identification by peptide mass fingerprinting.
The facility also has an in-house version of Mascot (Matrix Science) that provides customers with the option of loading their database to search for protein identification.
A Thermo Scientific Orbitrap Elite mass spectrometer is used for high-resolution and high-mass-accuracy analyses, that is coupled with a nano HPLC for proteomics work for analysis of complex mixtures.
In addition, we now offer Metabolomics, which began with the recent addition of a Bruker 12 T Solarix XR FTICR mass spectrometer purchased with National Institutes of Health, grant S10 OD025118 in 2019.
The instrument is capable of both electrospray and MALDI ionization, and contains multiple fragmentation modes, including electron capture disassociation (ECD), electron detachment disassociation (EDD), collision-induced disassociation (CID), electron transfer disassociation (ETD) and negative electron transfer disassociation (NTD).
The instrument can be interfaced with a Thermo Scientific Dionex Ultimate 3000 ultrahigh pressure liquid chromatograph (UHPLC) and data analysis will be assisted by Bruker MetaboScape software. Dr. Kevin Clark was hired September 2019 to spearhead the metabolomics portion of the facility.
Other instruments in the facility include Agilent GC-MS 5890, Bruker Esquire 3000 plus ion trap and Waters Micromass Q-TOF micro.
Presently, PAMMS is waiting delivery of a Bruker Impact II Q-TOF instrument with the Bruker Elute UHPLC system, which will provide additional capabilities to the facility.
Additional contact: Chau-Wen Chou