Bioexpression and Fermentation Facility
University of Georgia
Contact: Jeff Rapp, Ph.D., Director Bioexpression and Fermentation Facility
Phone: 706-542-1035
The BFF equipment is located across several lab spaces. Over 10,000 square feet of space has been allocated to the BFF in the Davison Life Sciences complex as well as an Annex Building solely for fermentation research. There are a number of ancillary rooms for cell culture, protein purification, monoclonal antibody production, and fermentation research as described below.
5500 sq. ft. laboratory and office space (Life Sciences building) Equipment (Protein Purification, Cell Culture, Monoclonal Antibody Production)
BioTek UV/VIS Microplate spectrophotometer
BioTek Elx405 plate washer (2)
Double-occupancy HEPA-filtered biosafety cabinets (BSC; 1, 6-foot)
Centrifuges (Refrigerated, 15,000 g) (3 units)
Scales (analytical gram and kilogram)
Microfuges
UV/VIS Spectrophotometer
Denovix DS-11 FX+ spectrophotometer
2 FPLCs and fraction collectors
3 ÄKTA Prime FPLCs
2 ÄKTA Start
1 ÄKTA Avant
1 ÄKTA Crossflow
1 Sartocon slice holder
Masterflex pumps
Electrophoresis Apparatus (DNA, SDS-PAGE, Westerns, and 2D)
Gradient and standard thermal cyclers
Sonicators (2)
Lyophilizers (2)
Cell Culture equipment
Double-occupancy HEPA-filtered biosafety cabinets (BSC; 3, 6-foot)
6, Air-jacketed CO2 incubators
Thermo Forma 3950 CO2 Reach-In incubator
BioFlo 310 Cell Culture Bioreactor
2 Kuhner ISF1-Z CO2 incubator shakers
Tabletop centrifuges
DasBox 250 mL x 4 bioreactors
4900 sq. ft. pilot plant (Annex Building)
1L Fermentation System (DASGIP), parallel bioprocessor and online monitoring (8 vessels)
2L Autoclavable Fermentation System
5L Autoclavable Fermentation System with Supervisory Control and Data Acquisition (SCADA, 6 vessels)
20L SIP Fermentor (Sartorius D-DCU Twin) with SCADA (2)
100L SIP Fermentor with SCADA
200L SIP Fermentor with SCADA
400L SIP Fermentor with SCADA
500L SIP Fermentor with SCADA
750L SIP Fermentor with SCADA
Secondary pumps for fed-batch substrate addition
2, Centrifuges (Refrigerated, 15,000 g)
Floor Shaker/Incubators (5)
Constant Systems Homogenizer (g cell lysis)
Niro Soavi Dynamic High Pressure homogenizer (kg cell lysis)
Electroporator
Chemical fume hood
Agilent 1260 HPLC with UV/VIS/fluorescent spectrophotometer and RI detection capability (2)
Shimadzu HPLC with UV Vis capability
Agilent Gas Chromatograph
YSI 2700 (fermentation product analysis)
SmartFlow 11000 TFF System (5 m2 or 10 m2 filter)
SmartFlow 3000 TFF System (0.5 m2 filter)
Millipore Pellicon TFF system (Cell culture processing)
Sharples Continuous tubular bowl centrifuges (3 units)
General facility equipment:
Freezers (-80 and -20°C)
Refrigerators
Microscopes (phase contrast, inverted, and dissection)
Autoclaves (large and small)
Walk-in cold room
Liquid N2 cryostorage freezer and dewars
Dishwasher
Computers for all personnel
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